I'm attempting to proactively thwart zero-day attacks on my network. Can I prevent zero-day attacks with a combination of good security practices, VLAN network segmentation and an NAC system?
As you probably know, it's never possible to completely prevent zero-day attacks. However, the controls you mention are a good start toward dramatically reducing the risks. Let's expand a bit on "good security practices" and look at some of the particular management practices that can lower your exposure to
Firewalls play a vital role in preventing zero-day attacks. Use them to protect the perimeter of your network from unsolicited traffic. You should also use host-based firewalls (such as Windows Firewall) to limit the inbound connections allowed to each system on your network. Ideally, most systems (e.g. workstations) will not allow any inbound connections.
Patch management is also critical. Many zero-day attacks are simply novel exploits of a previously known vulnerability. If you keep your operating systems and applications patched, you'll be immune from the vast majority of zero-day exploits.
Antivirus software and intrusion detection systems may not help with true zero-day attacks, but they play a valuable role in protecting your network from known issues. Don't neglect them when planning your security infrastructure.
I hope this helps you plan and implement your network security controls. Good luck in your battle to secure your enterprise information assets!
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